the Kingdoms of Living ThingsAchievement Test, comprising 35 multiple-choicequestions, was developed by the researcher. Opinions ofone biology education field expert and three biologyteachers about the questions’ scientific appropriateness andcontent validity were considered. Furthermore, twoTurkish language and literature teachers also examined thequestions in terms of language and expression. Based ontheir feedback, necessary adjustments were made to the test.The reliability investigation of the corrected and finalizedform of KLTAT was carried out with 176 tenth-gradestudents from an Anatolian high school.